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Expression of SLC16A1 in SW1088 and <t>HS683</t> after transfection with si-SLC16A1. A The expressions of LDHA and SLC16A1 in low-grade glioma cell lines. The X-axis represented different cells, while the Y-axis represented the relative expression of LDHA and SLC16A1. Control, normal human glial cells; Low-grade glioma cell lines SW1088 and HS683. **, P < 0.01, when compared with control. B The mRNA expression of SLC16A1 was detected by qRT-PCR. C The protein expression of SLC16A1 was detected by western blot. Protein concentrations were determined by BCA assay, and equal amounts (30 µg) were loaded in each lane.***, P < 0.001
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Expression of SLC16A1 in SW1088 and HS683 after transfection with si-SLC16A1. A The expressions of LDHA and SLC16A1 in low-grade glioma cell lines. The X-axis represented different cells, while the Y-axis represented the relative expression of LDHA and SLC16A1. Control, normal human glial cells; Low-grade glioma cell lines SW1088 and HS683. **, P < 0.01, when compared with control. B The mRNA expression of SLC16A1 was detected by qRT-PCR. C The protein expression of SLC16A1 was detected by western blot. Protein concentrations were determined by BCA assay, and equal amounts (30 µg) were loaded in each lane.***, P < 0.001

Journal: Discover Oncology

Article Title: Identification and validation of LDHA and SLC16A1 for predicting prognosis and diagnosis in lower-grade glioma

doi: 10.1007/s12672-025-03297-2

Figure Lengend Snippet: Expression of SLC16A1 in SW1088 and HS683 after transfection with si-SLC16A1. A The expressions of LDHA and SLC16A1 in low-grade glioma cell lines. The X-axis represented different cells, while the Y-axis represented the relative expression of LDHA and SLC16A1. Control, normal human glial cells; Low-grade glioma cell lines SW1088 and HS683. **, P < 0.01, when compared with control. B The mRNA expression of SLC16A1 was detected by qRT-PCR. C The protein expression of SLC16A1 was detected by western blot. Protein concentrations were determined by BCA assay, and equal amounts (30 µg) were loaded in each lane.***, P < 0.001

Article Snippet: The LGG cell lines of SW1088 and HS683 cell lines were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Expressing, Transfection, Control, Quantitative RT-PCR, Western Blot, BIA-KA

Low expression of SLC16A1 inhibits glioma cell proliferation, migration, and invasion. A Proliferation curve of SW1088 cells. B Proliferation curve of HS683 cells. C Transwell assay was used to detect the migration ability of SW1088 and HS683 cells (×100). D Transwell assay was used to detect the invasion ability of SW1088 and HS683 cells (×100). *, P < 0.05, **, P < 0.01, ***, P < 0.001

Journal: Discover Oncology

Article Title: Identification and validation of LDHA and SLC16A1 for predicting prognosis and diagnosis in lower-grade glioma

doi: 10.1007/s12672-025-03297-2

Figure Lengend Snippet: Low expression of SLC16A1 inhibits glioma cell proliferation, migration, and invasion. A Proliferation curve of SW1088 cells. B Proliferation curve of HS683 cells. C Transwell assay was used to detect the migration ability of SW1088 and HS683 cells (×100). D Transwell assay was used to detect the invasion ability of SW1088 and HS683 cells (×100). *, P < 0.05, **, P < 0.01, ***, P < 0.001

Article Snippet: The LGG cell lines of SW1088 and HS683 cell lines were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Expressing, Migration, Transwell Assay